Instructors:
Dr. Teryl Frey
Dr. Margo Brinton
Office:
418 Science Annex
414 Science Annex
Office Phone:
651-3105
651-3113
Email:
tfrey@gsu.edu
biomab@panther.gsu.edu
Home Page:
www.gsu.edu/~biotkf
Office Hours:
By appointment
By appointment
Lecture: 4:30-7:00 PM,
Tuesdays
251 Sparks
Laboratory: 7:20-9:40 PM, Tuesdays
334 Natural Science Center
Text: Medical Virology, Fourth Edition, by D.O. White and F.J. Fenner, Academic Press
Lecture Notes: Lecture notes will be availalbe on the instructors' home
pages for a period of one week prior through one
week after the specific lecture.
Laboratory Manual: The lab manual will be distributed in the lab.
A BLUE ($20) SUPPLY CARD IS REQUIRED FOR THE LAB. CARDS CAN BE PURCHASED AT THE GSU BOOKSTORE.
Grading: 500 possible points
Exam I (Feb. 15)
(100 points)
Exam II (March 29) (100
points)
Exam III (Tues., May 2) (100 points)
Laboratory Grade (200 points)
Grade Scale: A = 90-100; B= 80-89; C = 70-79; D = 60-69; F <60
Examinations: will be 1½ hours in length and will consist of 50% short answer, definition and true-false questions and 50% essay questions. To assist students in preparation for the exams, one week before each exam a set of study questions from the lecture material will be available on the instructors' home pages. Some of these questions will appear on the exam. Each exam will cover only material presented since the previous exam; none of the exams will be cumulative in nature.
Make-up exam policy: One make-up exam will be allowed if a valid excuse is presented before the scheduled exam time. The make-up exam will be given on Tuesday, April 25, and will consist of material covered on both Exams I and II.
Laboratory grade: The laboratory grade will be based on performance on the laboratory notebook and laboratory exam as well as attendance. These requirements will be discussed in the laboratory. The first meeting of the laboratory is Tuesday, Jan. 11. At the second meeting, the supply card must be given to the laboratory instructors.
TENTATIVE SCHEDULE
DATE TOPIC TEXT ASSIGNMENT
Jan. 11
Introduction to Virology
Chapter 1
Virion Structure
Mar. 3
LAST DAY TO WITHDRAW AND RECEIVE A W
Biology 4575
Spring Semester 2000
Laboratory Guidelines
Laboratory Times: Thursdays, 7:20-9:50 PM
Classroom:
334 Natural Science Center
Laboratory Manual: The lab manual will be distributed in the lab. Students should read the lab procedures before coming to class!
A SUPPLY CARD #2 ($20) IS REQUIRED. CARDS CAN BE PURCHASED AT THE GSU BOOKSTORE. STUDENTS MUST PRESENT THIS CARD TO THE LAB INSTRUCTORS BY THE BEGINNING OF THE JAN. 18 LAB PERIOD. STUDENTS NOT DOING SO WILL NOT BE ALLOWED TO ATTEND THE LAB.
Grading: The laboratory will factor into the student's final grade in two ways:
Two Exams each worth 50 points given on Feb. 29 and April 25
In-class performance as assessed by attendance and maintenance of a lab notebook will contribute 100 points to the total grade.
Attendance at laboratory sessions is required. One excused absence is permitted if approved by the laboratory instructors. Each unexcused absence or additional excused absence will result in the deduction of 10 points from the in class performance grade.
Laboratory Notebook: Each laboratory will be written up in the following format:
I. Introduction- (one page maximum). This section will present a concise background about the specific exercise/experiment.
II. Materials and Methods- The section will cover the methods used. The lab manual description can be used in preparation of this section, but be sure to indicate any changes in procedure that are made.
III. Results- (one page maximum). This section should contain only results, not interpretations of results (save interpretations for the Discussion section).
IV. Discussion- (one to two pages). This section will consist
of interpretations of your results. Discuss the significance of the
exercise/experiment performed.
Laboratory notebooks will be turned in for grading twice during the semester: Feb. 29 and April 25. Late submission of the lab notebook will result in a 10 point deduction from the laboratory grade.
LABORATORY SCHEDULE
DATE TOPIC
Jan. 11 Introduction
Jan. 18
Lab 1. Virus Quantitation and Plaque Assay
SUPPLY CARDS DUE
Jan. 25 Lab 2. Virus Growth and Purification (Part 1)
Feb. 1 Lab 2. Virus Growth and Purification (Part 2)
Feb. 8 Lab 2. Virus Growth and Purification (Part 3)
Feb. 15 Lab 3. Electrophoresis of Virus Proteins
Feb. 22
Electron Microscopy of Viruses presented by Dr. Robert Simmons, GSU
(No lab notebook report necessary)
Feb. 29
Lab Notebooks Due for Labs 1-3
Lab Exam I
Mar. 7 Spring Break
March 14 Lab 4. PCR for Virus Identification (Part I)
March 21 Lab 4. PCR for Virus Identification (Part II)
March 28 Lab 5. Cell Culture
April 4 Lab 6. Serology: Hemagglutination Inhibition
April 11 Lab 7. Serology: ELISA
April 18 Lab 8. Serology: Latex Agglutination
April 25
Lab Notebooks Due for Labs 4-8
Lab Exam II