1.  (4 pts.)  BRIEFLY, list the steps in the procedure of immunofluorescence microscopy.

    1.
 
 
 
 

WHY IS IT USED TO STUDY CELL COMPONENTS INSTEAD OF JUST USING A LIGHT MICROSCOPE?
 
 

2. (4 pts.) List all of the functions that require ATP during muscle contraction and relaxation
 
 
 

3. (6 pts.) a.  Draw a picture of a crawling cell - also draw in the actin filaments
 
 
 
 
 
 

b.  Draw how the picture would look if cytocholasin B was added to the cell above.
 
 
 
 
 

c.  Draw how the picture would look if all myosin was missing from the cell.
 
 
 
 
 
 

4. (2 pts.)  Name 2 pieces of experimental evidence that led to the deduction of the chemical and physical properties of DNA.

    1.
 

    2.
 

5. (2 pts.) There are several genetic diseases that arise when an individual has a defective helicase protein.  What sorts of problems (i.e. what does not work right) would these people have?
 
 
 

6.  (2 pts.) What can RNA polymerase do that DNA polymerase cannot do?
 
 

7.  (5 pts.)  Make a drawing of DNA replication, showing both sides of a replication bubble.  Be sure to label leading and lagging strands on both sides.  Be sure to include any major proteins (and label them).